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jd633 vector  (Addgene inc)


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    Addgene inc jd633 vector
    Jd633 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jd633 vector/product/Addgene inc
    Average 93 stars, based on 17 article reviews
    jd633 vector - by Bioz Stars, 2026-02
    93/100 stars

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    T2 CRISPR plants were genotyped to detect the presence of Cas9 using primers designed around the first 800 BP of the Cas9 sequence used in the trasnformation vector JD633 (Addgene). Untransformed wildtype Bd21-3 plants were also used as controls as well as water as a negative control. Bands indicate presence of Cas9 within T2 plants.

    Journal: bioRxiv

    Article Title: Forward Genetic Stomatal CO 2 Response Screen in Grass Brachypodium distachyon Reveals Central Function of a MAP Kinase in Early Stomatal CO 2 Signal Transduction

    doi: 10.1101/2023.09.28.559842

    Figure Lengend Snippet: T2 CRISPR plants were genotyped to detect the presence of Cas9 using primers designed around the first 800 BP of the Cas9 sequence used in the trasnformation vector JD633 (Addgene). Untransformed wildtype Bd21-3 plants were also used as controls as well as water as a negative control. Bands indicate presence of Cas9 within T2 plants.

    Article Snippet: Oligos were phosphorylated and utilized in a Golden Gate Reaction to be ligated into the CRISPR destination vector JD633 (Addgene).

    Techniques: CRISPR, Sequencing, Plasmid Preparation, Negative Control